Posts tagged with method
FlexiBAC, our open source protein expression system
One of great things here at the MPI-CBG are our Service and Facilities, among them the protein expression facility (PEPC), which has been heavily used by us in recent years. In collaboration with Jeff Woodruff, a former postdoc, they developed FlexiBAC, an amazingly versatile protein expression system in insect cells, which was developed with a huge range of different constructs and tags, which might fit your needs as well.
Rheology of protein condensates using optical traps
Louise and her coworkers now developed a novel method based on optical traps to study the frequency-dependent rheology and the surface tension of P-granule protein PGL-3 condensates as a function of salt concentration.
Check out our new user’s guide for phase separation assays with purified proteins!
The formation of membrane-less organelles and compartments by protein phase separation is an important way in which cells organize their cytoplasm and nucleoplasm. In vitro phase separation assays with purified proteins have become the standard way to investigate proteins that form membrane-less compartments.