Lab methods

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  • Actin Prep from Brains
  • Coupling IgG to Affiprep Beads
  • Expression, purification, denaturation, and renaturation of T7Δ90
  • Making p50 Dynamitin
  • NHS-Rhodamine labeling of Actin
  • Affinity purification of antisera or antigen
  • Ammonium sulfate precipitation
  • Antigen design and administration
  • General GST expression
  • Removing HSPs bound to E. coli fusion proteins
  • Silver staining
  • TCA precipitation of proteins
  • Biolistic transformation of c.elegans (bombardment protocol)
  • C. elegans - Basics
  • C. elegans - Biochemistry
  • Cenix protocol for production of large scale dsRNA
  • C. elegans dissection for embryo analysis
  • C. elegans embryo purification and embryo extract generation
  • C. elegans - Immunostaining
  • C. elegans techniques- Protein biochemistry
  • dsRNA Production for RNAi
  • Easy worm western blot using the iBlot
  • Fluorescence correlation spectroscopy (FCS) in C. elegans
  • Growing worms on plates
  • Making Agarose Pads
  • RNAi by feeding
  • RNAi by soaking
  • Whole-worm immunoprecipitation
  • Centrosome Prep
  • CHO Centrosome Prep
  • His-hsAurora Kinase A purification protocol
  • Labeling Tubulin and Quantifying Labeling Stoichiometry
  • Preparation of Tubulin from Porcine Brain
  • TACC3 purification protocol
  • XMAP215-GFP purification protocol
  • ZYG9-GFP purification protocol
  • Microtubule Dynamics by Fluorescence Microscopy in Xenopus egg extracts
  • Preparation of DNA beads