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Methods

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General Methods

  • Affinity Purification of antisera on antigen
  • Ammonium Sulfate Precipitation
  • Antigen design and administration
  • BAC Modification (GeneBridges, with Tips from Alex B)
  • Counter-Selection BAC Modification (GeneBridges, with Tips from Alex B)
  • DMSO-Competent E. coli cells
  • DNA Precipitation
  • Electrocompetent Bacteria
  • General GST expression
  • Immunization and Antibody Purification Protocols
  • How to Write a Paper
  • Removing HSPs bound to E. coli fusion proteins
  • Silver Staining
  • Standard Blotting Procedure
  • Standard Blotting Procedure
  • The Slacker Way (kit free) for Making Fusion Proteins for Antibody Generation/Affinity Purification
  • Transformation of DMSO-Competent E. coli
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