LOCUS       R6Kamp_LAP_GFP_         4238 bp ss-DNA     circular SYN 17-APR-2008
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      -
COMMENT     ApEinfo:methylated:0
FEATURES             Location/Qualifiers
     rep_origin      34..414
                     /label=R6K ori (pirate)
                     /note=ApEinfo_fwdcolor=#006868
                     /note=ApEinfo_revcolor=#006868
     promoter        544..579
                     /label=Bact.prom. from pEGFP-N3
                     /note=ApEinfo_fwdcolor=cyan
                     /note=ApEinfo_revcolor=green
     CDS             812..1471
                     /label=AmpR
                     /note=ApEinfo_fwdcolor=yellow
                     /note=ApEinfo_revcolor=yellow
     misc_structure  1565..1585
                     /label=TEV-site
                     /note=ApEinfo_fwdcolor=cyan
                     /note=ApEinfo_revcolor=cyan
     protein_bind    1598..1642
                     /label=S-peptide
                     /note=ApEinfo_fwdcolor=#00ffff
                     /note=ApEinfo_revcolor=#00ffff
     misc_feature    1676..1711
                     /label=PreScission
                     /note=ApEinfo_fwdcolor=cyan
                     /note=ApEinfo_revcolor=cyan
     misc_feature    1682..1705
                     /label=PreScission(1)
                     /note=ApEinfo_label=PreScission
                     /note=ApEinfo_fwdcolor=#717dff
                     /note=ApEinfo_revcolor=#717dff
     misc_feature    1733..2449
                     /label=eGFP mammalian
                     /note=ApEinfo_fwdcolor=#37ff42
                     /note=ApEinfo_revcolor=#37ff42
     misc_feature    2477..3062
                     /label=IRES
                     /note=ApEinfo_fwdcolor=#ffb54e
                     /note=ApEinfo_revcolor=#ffb54e
     promoter        3076..3156
                     /label=gb3-prom
                     /note=ApEinfo_fwdcolor=#ff42ce
                     /note=ApEinfo_revcolor=#ff42ce
     CDS             3169..3960
                     /label=Kan
                     /note=neoR
                     /note=ApEinfo_fwdcolor=yellow
                     /note=ApEinfo_revcolor=yellow
BASE COUNT      1049 A      1124 C      1123 G       942 T         0 OTHER
ORIGIN      ?
        1 CGGCCACGAT GCGTCCGGCG TAGAGGATCT GAAGATCAGC AGTTCAACCT GTTGATAGTA
       61 CGTACTAAGC TCTCATGTTT CACGTACTAA GCTCTCATGT TTAACGTACT AAGCTCTCAT
      121 GTTTAACGAA CTAAACCCTC ATGGCTAACG TACTAAGCTC TCATGGCTAA CGTACTAAGC
      181 TCTCATGTTT CACGTACTAA GCTCTCATGT TTGAACAATA AAATTAATAT AAATCAGCAA
      241 CTTAAATAGC CTCTAAGGTT TTAAGTTTTA TAAGAAAAAA AAGAATATAT AAGGCTTTTA
      301 AAGCTTTTAA GGTTTAACGG TTGTGGACAA CAAGCCAGGG ATGTAACGCA CTGAGAAGCC
      361 CTTAGAGCCT CTCAAAGCAA TTTTGAGTGA CACAGGAACA CTTAACGGCT GACATGGGAA
      421 TTAGCTTCAC GCTGCCGCAA GCACTCAGGG CGCAAGGGCT GCTAAAGGAA GCGGATAGAC
      481 GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT
      541 ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG
      601 AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC
      661 ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA
      721 TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA
      781 GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG
      841 CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC
      901 TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC
      961 AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT
     1021 TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA
     1081 TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG
     1141 TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT
     1201 ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG
     1261 ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG
     1321 TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT
     1381 CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC
     1441 TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACCCAGC CCGCCTAATG AGCGGGCTTT
     1501 TTTTTGAACA AAAGGCTGCA GCTGGACTTC TAGAGCTGAT TATGATATTC CAACTACTGC
     1561 AAGCGAGAAT CTTTATTTTC AGGGCGCCGC CAAATTCAAA GAAACCGCTG CTGCTAAATT
     1621 CGAACGCCAG CACATGGACA GCGGAGGTGG AGGTTCGAGC GGTCCCTCGG GTTCGTCGAG
     1681 CCTGGAAGTT CTGTTCCAGG GGCCCCTGTC GTCGAGCGGT CCCTCGGGTT CGATGGTGAG
     1741 CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT
     1801 AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT
     1861 GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC
     1921 CACCCTGACC TACGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA
     1981 CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA
     2041 CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG
     2101 CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA
     2161 GTACAACTAC AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA ACGGCATCAA
     2221 GGTGAACTTC AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA
     2281 CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG
     2341 CACCCAGTCC GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTTAAGGA
     2401 GTTCGTGACC GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGT AATGATCTAG
     2461 AGTCGAGTTA ATTAAGAATT CCGCCCCCCC CCTCTCCCTC CCCCCCCCCT AACGTTACTG
     2521 GCCGAAGCCG CTTGGAATAA GGCCGGTGTG CGTTTGTCTA TATGTTATTT TCCACCATAT
     2581 TGCCGTCTTT TGGCAATGTG AGGGCCCGGA AACCTGGCCC TGTCTTCTTG ACGAGCATTC
     2641 CTAGGGGTCT TTCCCCTCTC GCCAAAGGAA TGCAGGGTCT GTTGAATGTC GTGAAGGAAG
     2701 CAGTTCCTCT GGAAGCTTCT TGAAGACAAA CAACGTCTGT AGCGACCCTT TGCAGGCAGC
     2761 GGAACCCCCC ACCTGGCGAC AGGTGCCTCT GCGGCCAAAA GCCACGTGTA TAAGATACAC
     2821 CTGCAAAGGC GGCACAACCC CAGTGCCACG TTGTGAGTTG GATAGTTGTG GAAAGAGTCA
     2881 AATGGCTCTC CTCAAGCGTA TTCAACAAGG GGCTGAAGGA TGCCCAGAAG GTACCCCATT
     2941 GTATGGGATC TGATCTGGGG CCTCGGTGCA CATGCTTTAC ATGTGTTTAG TCGAGGTTAA
     3001 AAAAACGTCT AGGCCCCCCG AACCACGGGG ACGTGGTTTT CCTTTGAAAA ACACGATGAT
     3061 AATATGGCCA CAACCTGTTA CATTGCACAA GATAAAAATA TATCATCACG AACAGTAAAA
     3121 CTGTCTGCTT ACATAAACAG TAATACAAGG GGTGTTATGG GATCGGCCAT TGAACAAGAT
     3181 GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA
     3241 CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG
     3301 GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG
     3361 CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT
     3421 GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT
     3481 CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG
     3541 CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT
     3601 ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC
     3661 GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC
     3721 GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA
     3781 TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC
     3841 CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT
     3901 ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA
     3961 CTCGAGTTGC CCTCATCTGT TACGCCGGCG GTAGCCGGCC AGCCTCGCAG AGCAGGATTC
     4021 CCGTTGAGCA CCGCCAGGTG CGAATAAGGG ACAGTGAAGA AGGAACACCC GCTCGCGGGT
     4081 GGGCCTACTT CACCTATCCT GCCCGGCTGA CGCCGTTGGA TACACCAAGG AAAGTCTACA
     4141 CGAACCCTTT GGCAAAATCC TGTATATCGT GCGAAAAAGG ATGGATATAC CGAAAAAATC
     4201 GCTATAATGA CCCCGAAGCA GGGTTATGCA GCGGAAAA
//