LOCUS       R6Kamp_LAP_Cherr        4230 bp ss-DNA     circular SYN 17-APR-2008
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      -
COMMENT     ApEinfo:methylated:0
FEATURES             Location/Qualifiers
     rep_origin      34..414
                     /label=R6K ori (pirate)
                     /note=ApEinfo_fwdcolor=#006868
                     /note=ApEinfo_revcolor=#006868
     promoter        544..579
                     /label=Bact.prom. from pEGFP-N3
                     /note=ApEinfo_fwdcolor=cyan
                     /note=ApEinfo_revcolor=green
     CDS             812..1471
                     /label=AmpR
                     /note=ApEinfo_fwdcolor=yellow
                     /note=ApEinfo_revcolor=yellow
     misc_structure  1565..1585
                     /label=TEV-site
                     /note=ApEinfo_fwdcolor=cyan
                     /note=ApEinfo_revcolor=cyan
     protein_bind    1598..1642
                     /label=S-peptide
                     /note=ApEinfo_fwdcolor=#00ffff
                     /note=ApEinfo_revcolor=#00ffff
     misc_feature    1676..1711
                     /label=PreScission
                     /note=ApEinfo_fwdcolor=cyan
                     /note=ApEinfo_revcolor=cyan
     misc_feature    1682..1705
                     /label=PreScission(1)
                     /note=ApEinfo_label=PreScission
                     /note=ApEinfo_fwdcolor=#717dff
                     /note=ApEinfo_revcolor=#717dff
     gene            1733..2440
                     /label=mCherry(human)
                     /note=ApEinfo_fwdcolor=#ff5766
                     /note=ApEinfo_revcolor=#ff5766
     misc_feature    join(2468..2478,2483..3054)
                     /label=IRES(mut)
                     /note=ApEinfo_fwdcolor=#ffc03c
                     /note=ApEinfo_revcolor=#ffc03c
     promoter        3068..3148
                     /label=gb3-prom
                     /note=ApEinfo_fwdcolor=#ff42ce
                     /note=ApEinfo_revcolor=#ff42ce
     CDS             3161..3952
                     /label=Kan
                     /note=neoR
                     /note=ApEinfo_fwdcolor=yellow
                     /note=ApEinfo_revcolor=yellow
BASE COUNT      1038 A      1111 C      1141 G       940 T         0 OTHER
ORIGIN      ?
        1 CGGCCACGAT GCGTCCGGCG TAGAGGATCT GAAGATCAGC AGTTCAACCT GTTGATAGTA
       61 CGTACTAAGC TCTCATGTTT CACGTACTAA GCTCTCATGT TTAACGTACT AAGCTCTCAT
      121 GTTTAACGAA CTAAACCCTC ATGGCTAACG TACTAAGCTC TCATGGCTAA CGTACTAAGC
      181 TCTCATGTTT CACGTACTAA GCTCTCATGT TTGAACAATA AAATTAATAT AAATCAGCAA
      241 CTTAAATAGC CTCTAAGGTT TTAAGTTTTA TAAGAAAAAA AAGAATATAT AAGGCTTTTA
      301 AAGCTTTTAA GGTTTAACGG TTGTGGACAA CAAGCCAGGG ATGTAACGCA CTGAGAAGCC
      361 CTTAGAGCCT CTCAAAGCAA TTTTGAGTGA CACAGGAACA CTTAACGGCT GACATGGGAA
      421 TTAGCTTCAC GCTGCCGCAA GCACTCAGGG CGCAAGGGCT GCTAAAGGAA GCGGATAGAC
      481 GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT
      541 ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG
      601 AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC
      661 ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA
      721 TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA
      781 GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG
      841 CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC
      901 TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC
      961 AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT
     1021 TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA
     1081 TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG
     1141 TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT
     1201 ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG
     1261 ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG
     1321 TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT
     1381 CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC
     1441 TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACCCAGC CCGCCTAATG AGCGGGCTTT
     1501 TTTTTGAACA AAAGGCTGCA GCTGGACTTC TAGAGCTGAT TATGATATTC CAACTACTGC
     1561 AAGCGAGAAT CTTTATTTTC AGGGCGCCGC CAAATTCAAA GAAACCGCTG CTGCTAAATT
     1621 CGAACGCCAG CACATGGACA GCGGAGGTGG AGGTTCGAGC GGTCCCTCGG GTTCGTCGAG
     1681 CCTGGAAGTT CTGTTCCAGG GGCCCCTGTC GTCGAGCGGT CCCTCGGGTT CGATGGTGAG
     1741 CAAGGGCGAG GAGGATAACA TGGCCATCAT CAAGGAGTTC ATGCGCTTCA AGGTGCACAT
     1801 GGAGGGCTCC GTGAACGGCC ACGAGTTCGA GATCGAGGGC GAGGGCGAGG GCCGCCCCTA
     1861 CGAGGGCACC CAGACCGCCA AGCTGAAGGT GACCAAGGGT GGCCCCCTGC CCTTCGCCTG
     1921 GGACATCCTG TCCCCTCAGT TCATGTACGG CTCCAAGGCC TACGTGAAGC ACCCCGCCGA
     1981 CATCCCCGAC TACTTGAAGC TGTCCTTCCC CGAGGGCTTC AAGTGGGAGC GCGTGATGAA
     2041 CTTCGAGGAC GGCGGCGTGG TGACCGTGAC CCAGGACTCC TCCCTGCAGG ACGGCGAGTT
     2101 CATCTACAAG GTGAAGCTGC GCGGCACCAA CTTCCCCTCC GACGGCCCCG TAATGCAGAA
     2161 GAAGACCATG GGCTGGGAGG CCTCCTCCGA GCGGATGTAC CCCGAGGACG GCGCCCTGAA
     2221 GGGCGAGATC AAGCAGAGGC TGAAGCTGAA GGACGGCGGC CACTACGACG CTGAGGTCAA
     2281 GACCACCTAC AAGGCCAAGA AGCCCGTGCA GCTGCCCGGC GCCTACAACG TCAACATCAA
     2341 GTTGGACATC ACCTCCCACA ACGAGGACTA CACCATCGTG GAACAGTACG AACGCGCCGA
     2401 GGGCCGCCAC TCCACCGGCG GCATGGACGA GCTGTACAAG TAATGATCTA GAGTCGAGTT
     2461 AATTAAGAAT TCCGCCCCCC CCCCTCTCCC TCCCCCCCCC CTAACGTTAC TGGCCGAAGC
     2521 CGCTTGGAAT AAGGCCGGTG TGCGTTTGTC TATATGTTAT TTTCCACCAT ATTGCCGTCT
     2581 TTTGGCAATG TGAGGGCCCG GAAACCTGGC CCTGTCTTCT TGACGAGCAT TCCTAGGGGT
     2641 CTTTCCCCTC TCGCCAAAGG AATGCAGGGT CTGTTGAATG TCGTGAAGGA AGCAGTTCCT
     2701 CTGGAAGCTT CTTGAAGACA AACAACGTCT GTAGCGACCC TTTGCAGGCA GCGGAACCCC
     2761 CCACCTGGCG ACAGGTGCCT CTGCGGCCAA AAGCCACGTG TATAAGATAC ACCTGCAAAG
     2821 GCGGCACAAC CCCAGTGCCA CGTTGTGAGT TGGATAGTTG TGGAAAGAGT CAAATGGCTC
     2881 TCCTCAAGCG TATTCAACAA GGGGCTGAAG GATGCCCAGA AGGTACCCCA TTGTATGGGA
     2941 TCTGATCTGG GGCCTCGGTG CACATGCTTT ACATGTGTTT AGTCGAGGTT AAAAAAACGT
     3001 CTAGGCCCCC CGAACCACGG GGACGTGGTT TTCCTTTGAA AAACACGATG ATAATATGGC
     3061 CACAACCTGT TACATTGCAC AAGATAAAAA TATATCATCA CGAACAGTAA AACTGTCTGC
     3121 TTACATAAAC AGTAATACAA GGGGTGTTAT GGGATCGGCC ATTGAACAAG ATGGATTGCA
     3181 CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC
     3241 AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT
     3301 TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC
     3361 GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG
     3421 AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC
     3481 TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC
     3541 GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT
     3601 GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC
     3661 CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA
     3721 TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA
     3781 CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT
     3841 TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC
     3901 TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GACTCGAGTT
     3961 GCCCTCATCT GTTACGCCGG CGGTAGCCGG CCAGCCTCGC AGAGCAGGAT TCCCGTTGAG
     4021 CACCGCCAGG TGCGAATAAG GGACAGTGAA GAAGGAACAC CCGCTCGCGG GTGGGCCTAC
     4081 TTCACCTATC CTGCCCGGCT GACGCCGTTG GATACACCAA GGAAAGTCTA CACGAACCCT
     4141 TTGGCAAAAT CCTGTATATC GTGCGAAAAA GGATGGATAT ACCGAAAAAA TCGCTATAAT
     4201 GACCCCGAAG CAGGGTTATG CAGCGGAAAA
//