LOCUS       R6Kamp_LAP_paGFP        4235 bp ss-DNA     circular SYN 17-APR-2008
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      -
COMMENT     ApEinfo:methylated:0
FEATURES             Location/Qualifiers
     rep_origin      34..414
                     /label=R6K ori (pirate)
                     /note=ApEinfo_fwdcolor=#006868
                     /note=ApEinfo_revcolor=#006868
     promoter        544..579
                     /label=Bact.prom. from pEGFP-N3
                     /note=ApEinfo_fwdcolor=cyan
                     /note=ApEinfo_revcolor=green
     CDS             812..1471
                     /label=AmpR
                     /note=ApEinfo_fwdcolor=yellow
                     /note=ApEinfo_revcolor=yellow
     misc_structure  1565..1585
                     /label=TEV-site
                     /note=ApEinfo_fwdcolor=cyan
                     /note=ApEinfo_revcolor=cyan
     protein_bind    1598..1642
                     /label=S-peptide
                     /note=ApEinfo_fwdcolor=#00ffff
                     /note=ApEinfo_revcolor=#00ffff
     misc_feature    1676..1711
                     /label=PreScission
                     /note=ApEinfo_fwdcolor=cyan
                     /note=ApEinfo_revcolor=cyan
     misc_feature    1682..1705
                     /label=PreScission(1)
                     /note=ApEinfo_label=PreScission
                     /note=ApEinfo_fwdcolor=#717dff
                     /note=ApEinfo_revcolor=#717dff
     gene            1733..2452
                     /label=paGFP(human)
                     /note=ApEinfo_fwdcolor=#50ffb5
                     /note=ApEinfo_revcolor=#50ffb5
     RBS             2477..3056
                     /label=IRES
                     /note=ApEinfo_fwdcolor=#ffb136
                     /note=ApEinfo_revcolor=#ffb136
     promoter        3070..3150
                     /label=gb3-prom
                     /note=ApEinfo_fwdcolor=#ff42ce
                     /note=ApEinfo_revcolor=#ff42ce
     CDS             3163..3954
                     /label=Kan
                     /note=neoR
                     /note=ApEinfo_fwdcolor=yellow
                     /note=ApEinfo_revcolor=yellow
BASE COUNT      1050 A      1120 C      1123 G       942 T         0 OTHER
ORIGIN      ?
        1 CGGCCACGAT GCGTCCGGCG TAGAGGATCT GAAGATCAGC AGTTCAACCT GTTGATAGTA
       61 CGTACTAAGC TCTCATGTTT CACGTACTAA GCTCTCATGT TTAACGTACT AAGCTCTCAT
      121 GTTTAACGAA CTAAACCCTC ATGGCTAACG TACTAAGCTC TCATGGCTAA CGTACTAAGC
      181 TCTCATGTTT CACGTACTAA GCTCTCATGT TTGAACAATA AAATTAATAT AAATCAGCAA
      241 CTTAAATAGC CTCTAAGGTT TTAAGTTTTA TAAGAAAAAA AAGAATATAT AAGGCTTTTA
      301 AAGCTTTTAA GGTTTAACGG TTGTGGACAA CAAGCCAGGG ATGTAACGCA CTGAGAAGCC
      361 CTTAGAGCCT CTCAAAGCAA TTTTGAGTGA CACAGGAACA CTTAACGGCT GACATGGGAA
      421 TTAGCTTCAC GCTGCCGCAA GCACTCAGGG CGCAAGGGCT GCTAAAGGAA GCGGATAGAC
      481 GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT
      541 ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG
      601 AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC
      661 ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA
      721 TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA
      781 GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG
      841 CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC
      901 TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC
      961 AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT
     1021 TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA
     1081 TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG
     1141 TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT
     1201 ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG
     1261 ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG
     1321 TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT
     1381 CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC
     1441 TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACCCAGC CCGCCTAATG AGCGGGCTTT
     1501 TTTTTGAACA AAAGGCTGCA GCTGGACTTC TAGAGCTGAT TATGATATTC CAACTACTGC
     1561 AAGCGAGAAT CTTTATTTTC AGGGCGCCGC CAAATTCAAA GAAACCGCTG CTGCTAAATT
     1621 CGAACGCCAG CACATGGACA GCGGAGGTGG AGGTTCGAGC GGTCCCTCGG GTTCGTCGAG
     1681 CCTGGAAGTT CTGTTCCAGG GGCCCCTGTC GTCGAGCGGT CCCTCGGGTT CGATGGTGAG
     1741 CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT
     1801 AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT
     1861 GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC
     1921 CACCTTCAGC TACGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA
     1981 CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA
     2041 CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG
     2101 CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA
     2161 GTACAACTAC AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA ACGGCATCAA
     2221 GGCCAACTTC AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA
     2281 CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG
     2341 CCACCAGTCC AAGCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA
     2401 GTTCGTGACC GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGT AATGATCTAG
     2461 AGTCGAGTTA ATTAAGAATT CCGCCCCTCT CCCTCCCCCC CCCCTAACGT TACTGGCCGA
     2521 AGCCGCTTGG AATAAGGCCG GTGTGCGTTT GTCTATATGT TATTTTCCAC CATATTGCCG
     2581 TCTTTTGGCA ATGTGAGGGC CCGGAAACCT GGCCCTGTCT TCTTGACGAG CATTCCTAGG
     2641 GGTCTTTCCC CTCTCGCCAA AGGAATGCAA GGTCTGTTGA ATGTCGTGAA GGAAGCAGTT
     2701 CCTCTGGAAG CTTCTTGAAG ACAAACAACG TCTGTAGCGA CCCTTTGCAG GCAGCGGAAC
     2761 CCCCCACCTG GCGACAGGTG CCTCTGCGGC CAAAAGCCAC GTGTATAAGA TACACCTGCA
     2821 AAGGCGGCAC AACCCCAGTG CCACGTTGTG AGTTGGATAG TTGTGGAAAG AGTCAAATGG
     2881 CTCTCCTCAA GCGTATTCAA CAAGGGGCTG AAGGATGCCC AGAAGGTACC CCATTGTATG
     2941 GGATCTGATC TGGGGCCTCG GTGCACATGC TTTACATGTG TTTAGTCGAG GTTAAAAAAC
     3001 GTCTAGGCCC CCCGAACCAC GGGGACGTGG TTTTCCTTTG AAAAACACGA TGATAATATG
     3061 GCCACAACCT GTTACATTGC ACAAGATAAA AATATATCAT CACGAACAGT AAAACTGTCT
     3121 GCTTACATAA ACAGTAATAC AAGGGGTGTT ATGGGATCGG CCATTGAACA AGATGGATTG
     3181 CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG
     3241 ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT
     3301 TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA
     3361 TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG
     3421 GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT
     3481 GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT
     3541 CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG
     3601 ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA
     3661 GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC
     3721 CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC
     3781 GACTGTGGCC GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT
     3841 ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC
     3901 GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAAGCCTC
     3961 GAGTTGCCCT CATCTGTTAC GCCGGCGGTA GCCGGCCAGC CTCGCAGAGC AGGATTCCCG
     4021 TTGAGCACCG CCAGGTGCGA ATAAGGGACA GTGAAGAAGG AACACCCGCT CGCGGGTGGG
     4081 CCTACTTCAC CTATCCTGCC CGGCTGACGC CGTTGGATAC ACCAAGGAAA GTCTACACGA
     4141 ACCCTTTGGC AAAATCCTGT ATATCGTGCG AAAAAGGATG GATATACCGA AAAAATCGCT
     4201 ATAATGACCC CGAAGCAGGG TTATGCAGCG GAAAA
//