LOCUS       R6Kamp_LAP_T2A_N        3684 bp ss-DNA     circular SYN 17-APR-2008
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      -
COMMENT     ApEinfo:methylated:0
FEATURES             Location/Qualifiers
     rep_origin      34..414
                     /label=R6K ori (pirate)
                     /note=ApEinfo_fwdcolor=#006868
                     /note=ApEinfo_revcolor=#006868
     promoter        544..579
                     /label=Bact.prom. from pEGFP-N3
                     /note=ApEinfo_fwdcolor=cyan
                     /note=ApEinfo_revcolor=green
     CDS             812..1471
                     /label=AmpR
                     /note=ApEinfo_fwdcolor=yellow
                     /note=ApEinfo_revcolor=yellow
     misc_structure  1565..1585
                     /label=TEV-site
                     /note=ApEinfo_fwdcolor=cyan
                     /note=ApEinfo_revcolor=cyan
     protein_bind    1598..1642
                     /label=S-peptide
                     /note=ApEinfo_fwdcolor=#00ffff
                     /note=ApEinfo_revcolor=#00ffff
     misc_feature    1676..1711
                     /label=PreScission
                     /note=ApEinfo_fwdcolor=cyan
                     /note=ApEinfo_revcolor=cyan
     misc_feature    1682..1705
                     /label=PreScission(1)
                     /note=ApEinfo_label=PreScission
                     /note=ApEinfo_fwdcolor=#717dff
                     /note=ApEinfo_revcolor=#717dff
     misc_feature    1733..2449
                     /label=eGFP mammalian
                     /note=ApEinfo_fwdcolor=#37ff42
                     /note=ApEinfo_revcolor=#37ff42
     misc_feature    2456..2509
                     /label=T2A
                     /note=ApEinfo_fwdcolor=cyan
                     /note=ApEinfo_revcolor=cyan
     promoter        2519..2599
                     /label=gb3-prom
                     /note=ApEinfo_fwdcolor=#ff42ce
                     /note=ApEinfo_revcolor=#ff42ce
     CDS             2612..3403
                     /label=Kan
                     /note=neoR
                     /note=ApEinfo_fwdcolor=yellow
                     /note=ApEinfo_revcolor=yellow
BASE COUNT       918 A       975 C       990 G       801 T         0 OTHER
ORIGIN      ?
        1 CGGCCACGAT GCGTCCGGCG TAGAGGATCT GAAGATCAGC AGTTCAACCT GTTGATAGTA
       61 CGTACTAAGC TCTCATGTTT CACGTACTAA GCTCTCATGT TTAACGTACT AAGCTCTCAT
      121 GTTTAACGAA CTAAACCCTC ATGGCTAACG TACTAAGCTC TCATGGCTAA CGTACTAAGC
      181 TCTCATGTTT CACGTACTAA GCTCTCATGT TTGAACAATA AAATTAATAT AAATCAGCAA
      241 CTTAAATAGC CTCTAAGGTT TTAAGTTTTA TAAGAAAAAA AAGAATATAT AAGGCTTTTA
      301 AAGCTTTTAA GGTTTAACGG TTGTGGACAA CAAGCCAGGG ATGTAACGCA CTGAGAAGCC
      361 CTTAGAGCCT CTCAAAGCAA TTTTGAGTGA CACAGGAACA CTTAACGGCT GACATGGGAA
      421 TTAGCTTCAC GCTGCCGCAA GCACTCAGGG CGCAAGGGCT GCTAAAGGAA GCGGATAGAC
      481 GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT
      541 ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG
      601 AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC
      661 ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA
      721 TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA
      781 GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG
      841 CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC
      901 TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC
      961 AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT
     1021 TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA
     1081 TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG
     1141 TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT
     1201 ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG
     1261 ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG
     1321 TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT
     1381 CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC
     1441 TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACCCAGC CCGCCTAATG AGCGGGCTTT
     1501 TTTTTGAACA AAAGGCTGCA GCTGGACTTC TAGAGCTGAT TATGATATTC CAACTACTGC
     1561 AAGCGAGAAT CTTTATTTTC AGGGCGCCGC CAAATTCAAA GAAACCGCTG CTGCTAAATT
     1621 CGAACGCCAG CACATGGACA GCGGAGGTGG AGGTTCGAGC GGTCCCTCGG GTTCGTCGAG
     1681 CCTGGAAGTT CTGTTCCAGG GGCCCCTGTC GTCGAGCGGT CCCTCGGGTT CGATGGTGAG
     1741 CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT
     1801 AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT
     1861 GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC
     1921 CACCCTGACC TACGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA
     1981 CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA
     2041 CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG
     2101 CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA
     2161 GTACAACTAC AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA ACGGCATCAA
     2221 GGTGAACTTC AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA
     2281 CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG
     2341 CACCCAGTCC GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTTAAGGA
     2401 GTTCGTGACC GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGG GCGCCGAGGG
     2461 CAGAGGAAGT CTTCTAACAT GCGGTGACGT GGAGGAGAAT CCCGGCCCTG CCACAACCTG
     2521 TTACATTGCA CAAGATAAAA ATATATCATC ACGAACAGTA AAACTGTCTG CTTACATAAA
     2581 CAGTAATACA AGGGGTGTTA TGGGATCGGC CATTGAACAA GATGGATTGC ACGCAGGTTC
     2641 TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG
     2701 CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC
     2761 CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC
     2821 CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG
     2881 GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA
     2941 GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG
     3001 CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG
     3061 TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT
     3121 CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC
     3181 CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG
     3241 GCTGGGTGTG GCGGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA
     3301 GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC
     3361 GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAAGCCTCG AGTTGCCCTC
     3421 ATCTGTTACG CCGGCGGTAG CCGGCCAGCC TCGCAGAGCA GGATTCCCGT TGAGCACCGC
     3481 CAGGTGCGAA TAAGGGACAG TGAAGAAGGA ACACCCGCTC GCGGGTGGGC CTACTTCACC
     3541 TATCCTGCCC GGCTGACGCC GTTGGATACA CCAAGGAAAG TCTACACGAA CCCTTTGGCA
     3601 AAATCCTGTA TATCGTGCGA AAAAGGATGG ATATACCGAA AAAATCGCTA TAATGACCCC
     3661 GAAGCAGGGT TATGCAGCGG AAAA
//