LOCUS       R6Kamp_LAP_T2A_B        3279 bp ss-DNA     circular SYN 17-APR-2008
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      -
COMMENT     ApEinfo:methylated:0
FEATURES             Location/Qualifiers
     rep_origin      34..414
                     /label=R6K ori (pirate)
                     /note=ApEinfo_fwdcolor=#006868
                     /note=ApEinfo_revcolor=#006868
     promoter        544..579
                     /label=Bact.prom. from pEGFP-N3
                     /note=ApEinfo_fwdcolor=cyan
                     /note=ApEinfo_revcolor=green
     CDS             812..1471
                     /label=AmpR
                     /note=ApEinfo_fwdcolor=yellow
                     /note=ApEinfo_revcolor=yellow
     misc_structure  1565..1585
                     /label=TEV-site
                     /note=ApEinfo_fwdcolor=cyan
                     /note=ApEinfo_revcolor=cyan
     protein_bind    1598..1642
                     /label=S-peptide
                     /note=ApEinfo_fwdcolor=#00ffff
                     /note=ApEinfo_revcolor=#00ffff
     misc_feature    1676..1711
                     /label=PreScission
                     /note=ApEinfo_fwdcolor=cyan
                     /note=ApEinfo_revcolor=cyan
     misc_feature    1682..1705
                     /label=PreScission(1)
                     /note=ApEinfo_label=PreScission
                     /note=ApEinfo_fwdcolor=#717dff
                     /note=ApEinfo_revcolor=#717dff
     misc_feature    1733..2449
                     /label=eGFP mammalian
                     /note=ApEinfo_fwdcolor=#37ff42
                     /note=ApEinfo_revcolor=#37ff42
     misc_feature    2456..2509
                     /label=T2A
                     /note=ApEinfo_fwdcolor=cyan
                     /note=ApEinfo_revcolor=cyan
     promoter        2519..2599
                     /label=gb3-prom
                     /note=ApEinfo_fwdcolor=#ff42ce
                     /note=ApEinfo_revcolor=#ff42ce
     misc_feature    2600..2998
                     /label=Bsd optimized
                     /note=ApEinfo_fwdcolor=#3bcfff
                     /note=ApEinfo_revcolor=#3bcfff
BASE COUNT       847 A       864 C       843 G       725 T         0 OTHER
ORIGIN      ?
        1 CGGCCACGAT GCGTCCGGCG TAGAGGATCT GAAGATCAGC AGTTCAACCT GTTGATAGTA
       61 CGTACTAAGC TCTCATGTTT CACGTACTAA GCTCTCATGT TTAACGTACT AAGCTCTCAT
      121 GTTTAACGAA CTAAACCCTC ATGGCTAACG TACTAAGCTC TCATGGCTAA CGTACTAAGC
      181 TCTCATGTTT CACGTACTAA GCTCTCATGT TTGAACAATA AAATTAATAT AAATCAGCAA
      241 CTTAAATAGC CTCTAAGGTT TTAAGTTTTA TAAGAAAAAA AAGAATATAT AAGGCTTTTA
      301 AAGCTTTTAA GGTTTAACGG TTGTGGACAA CAAGCCAGGG ATGTAACGCA CTGAGAAGCC
      361 CTTAGAGCCT CTCAAAGCAA TTTTGAGTGA CACAGGAACA CTTAACGGCT GACATGGGAA
      421 TTAGCTTCAC GCTGCCGCAA GCACTCAGGG CGCAAGGGCT GCTAAAGGAA GCGGATAGAC
      481 GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT
      541 ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG
      601 AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC
      661 ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA
      721 TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA
      781 GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG
      841 CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC
      901 TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC
      961 AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT
     1021 TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA
     1081 TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG
     1141 TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT
     1201 ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG
     1261 ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG
     1321 TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT
     1381 CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC
     1441 TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACCCAGC CCGCCTAATG AGCGGGCTTT
     1501 TTTTTGAACA AAAGGCTGCA GCTGGACTTC TAGAGCTGAT TATGATATTC CAACTACTGC
     1561 AAGCGAGAAT CTTTATTTTC AGGGCGCCGC CAAATTCAAA GAAACCGCTG CTGCTAAATT
     1621 CGAACGCCAG CACATGGACA GCGGAGGTGG AGGTTCGAGC GGTCCCTCGG GTTCGTCGAG
     1681 CCTGGAAGTT CTGTTCCAGG GGCCCCTGTC GTCGAGCGGT CCCTCGGGTT CGATGGTGAG
     1741 CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT
     1801 AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT
     1861 GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC
     1921 CACCCTGACC TACGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA
     1981 CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA
     2041 CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG
     2101 CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA
     2161 GTACAACTAC AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA ACGGCATCAA
     2221 GGTGAACTTC AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA
     2281 CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG
     2341 CACCCAGTCC GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTTAAGGA
     2401 GTTCGTGACC GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGG GCGCCGAGGG
     2461 CAGAGGAAGT CTTCTAACAT GCGGTGACGT GGAGGAGAAT CCCGGCCCTG CCACAACCTG
     2521 TTACATTGCA CAAGATAAAA ATATATCATC ACGAACAGTA AAACTGTCTG CTTACATAAA
     2581 CAGTAATACA AGGGGTGTTA TGGCTAAACC GCTCAGCCAG GAGGAATCTA CCCTGATCGA
     2641 ACGTGCCACC GCTACCATCA ACAGCATCCC GATCTCTGAG GACTATTCCG TGGCATCCGC
     2701 AGCTCTGTCT TCTGACGGCC GTATCTTCAC CGGCGTGAAC GTTTACCACT TTACTGGCGG
     2761 TCCGTGTGCA GAACTGGTGG TTCTCGGTAC TGCTGCTGCT GCTGCTGCTG GTAACCTGAC
     2821 CTGCATTGTC GCCATTGGCA ACGAAAACCG TGGTATCCTG TCTCCATGCG GTCGTTGTCG
     2881 TCAGGTTCTG CTGGATCTGC ATCCGGGCAT CAAAGCCATC GTCAAAGACT CTGATGGCCA
     2941 GCCGACTGCT GTTGGTATCC GTGAACTCCT GCCATCTGGC TACGTGTGGG AGGGTTAAAG
     3001 CCTCGAGTTG CCCTCATCTG TTACGCCGGC GGTAGCCGGC CAGCCTCGCA GAGCAGGATT
     3061 CCCGTTGAGC ACCGCCAGGT GCGAATAAGG GACAGTGAAG AAGGAACACC CGCTCGCGGG
     3121 TGGGCCTACT TCACCTATCC TGCCCGGCTG ACGCCGTTGG ATACACCAAG GAAAGTCTAC
     3181 ACGAACCCTT TGGCAAAATC CTGTATATCG TGCGAAAAAG GATGGATATA CCGAAAAAAT
     3241 CGCTATAATG ACCCCGAAGC AGGGTTATGC AGCGGAAAA
//