LOCUS       R6K_TY1_T2A_GFP_        4116 bp ss-DNA     circular SYN 17-APR-2008
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      -
COMMENT     ApEinfo:methylated:0
FEATURES             Location/Qualifiers
     CDS             199..858
                     /label=AmpR
                     /note=ApEinfo_fwdcolor=yellow
                     /note=ApEinfo_revcolor=yellow
     misc_feature    902..961
                     /label=2xTY1(c.e.opt)
                     /note=ApEinfo_fwdcolor=#ff3f3c
                     /note=ApEinfo_revcolor=#ff3f3c
     misc_feature    968..1021
                     /label=T2A
                     /note=ApEinfo_fwdcolor=cyan
                     /note=ApEinfo_revcolor=cyan
     misc_feature    1022..1738
                     /label=eGFP mammalian
                     /note=ApEinfo_fwdcolor=#3cff2d
                     /note=ApEinfo_revcolor=#3cff2d
     misc_feature    1743..1776
                     /label=FRT
                     /note=ApEinfo_fwdcolor=#ff8e19
                     /note=ApEinfo_revcolor=#ff8e19
     misc_feature    1931..2305
                     /label=RPSL
                     /note=ApEinfo_fwdcolor=#ff1524
                     /note=ApEinfo_revcolor=#ff1524
     CDS             2320..3111
                     /label=Kan
                     /note=neoR
                     /note=ApEinfo_fwdcolor=yellow
                     /note=ApEinfo_revcolor=yellow
     misc_feature    3119..3152
                     /label=FRT(1)
                     /note=ApEinfo_label=FRT
                     /note=ApEinfo_fwdcolor=#ff8e19
                     /note=ApEinfo_revcolor=#ff8e19
     misc_feature    3160..3225
                     /label=3xFLAG (c.e.opt)
                     /note=ApEinfo_fwdcolor=#ff3973
                     /note=ApEinfo_revcolor=#ff3973
     rep_origin      3537..3917
                     /label=R6K ori (pirate)
                     /note=ApEinfo_fwdcolor=#006868
                     /note=ApEinfo_revcolor=#006868
     promoter        4047..4082
                     /label=Bact.prom. from pEGFP-N3
                     /note=ApEinfo_fwdcolor=cyan
                     /note=ApEinfo_revcolor=green
BASE COUNT      1035 A      1074 C      1095 G       912 T         0 OTHER
ORIGIN      ?
        1 ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT
       61 GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA
      121 CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC
      181 GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC
      241 CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG
      301 GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA
      361 TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC
      421 GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT
      481 GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG
      541 CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT
      601 TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC
      661 TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT
      721 CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC
      781 ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC
      841 TCACTGATTA AGCATTGGTA ACCCAGCCCG CCTAATGAGC GGGCTTTTTT TTGAAAGCGC
      901 TGAAGTGCAT ACCAATCAGG ACCCGCTGGA CGAGGTTCAC ACAAACCAAG ATCCACTTGA
      961 TACTAGTGAG GGCAGAGGAA GTCTTCTAAC ATGCGGTGAC GTGGAGGAGA ATCCCGGCCC
     1021 TATGGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA
     1081 CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA
     1141 CGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC
     1201 CCTCGTGACC ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA
     1261 GCAGCACGAC TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT
     1321 CTTCAAGGAC GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT
     1381 GGTGAACCGC ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA
     1441 CAAGCTGGAG TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA
     1501 CGGCATCAAG GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC
     1561 CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA
     1621 CTACCTGAGC ACCCAGTCCG CCCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT
     1681 CCTGCTGGAG TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGACGAGC TGTACAAGTC
     1741 CGGAAGTTCC TATTCTCTAG AAAGTATAGG AACTTCGTGA ACCGGTGGTA CCGGCCTGGT
     1801 GATGATGGCG GGATCGTTGT ATATTTCTTG ACACCTTTTC GGCATCGCCC TAAAATTCGG
     1861 CGTCCTCATA TTGTGTGAGG ACGTTTTATT ACGTGTTTAC GAAGCAAAAG CTAAAACCAG
     1921 GAGCTATTTA ATGGCAACAG TTAACCAGCT GGTACGCAAA CCACGTGCTC GCAAAGTTGC
     1981 GAAAAGCAAC GTGCCTGCGC TGGAAGCATG CCCGCAAAAA CGTGGCGTAT GTACTCGTGT
     2041 ATATACTACC ACTCCTAAAA AACCGAACTC CGCGCTGCGT AAAGTATGCC GTGTTCGTCT
     2101 GACTAACGGT TTCGAAGTGA CTTCCTACAT CGGTGGTGAA GGTCACAACC TGCAGGAGCA
     2161 CTCCGTGATC CTGATCCGTG GCGGTCGTGT TAAAGACCTC CCGGGTGTTC GTTACCACAC
     2221 CGTACGTGGT GCGCTTGACT GCTCCGGCGT TAAAGACCGT AAGCAGGCTC GTTCCAAGTA
     2281 TGGCGTGAAG CGTCCTAAGG CTTAAGGAGG ACAATCATGA TTGAACAAGA TGGATTGCAC
     2341 GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA
     2401 ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT
     2461 GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG
     2521 TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA
     2581 AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT
     2641 CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG
     2701 GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG
     2761 GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC
     2821 GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT
     2881 GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC
     2941 TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT
     3001 GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT
     3061 CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGAATTCTGA
     3121 AGTTCCTATT CTCTAGAAAG TATAGGAACT TCGCTCGAGG ATTATAAAGA CCACGATGGT
     3181 GATTATAAAG ATCATGACAT CGACTACAAG GATGACGACG ACAAGAGCGC TTTGCCCTCA
     3241 TCTGTTACGC CGGCGGTAGC CGGCCAGCCT CGCAGAGCAG GATTCCCGTT GAGCACCGCC
     3301 AGGTGCGAAT AAGGGACAGT GAAGAAGGAA CACCCGCTCG CGGGTGGGCC TACTTCACCT
     3361 ATCCTGCCCG GCTGACGCCG TTGGATACAC CAAGGAAAGT CTACACGAAC CCTTTGGCAA
     3421 AATCCTGTAT ATCGTGCGAA AAAGGATGGA TATACCGAAA AAATCGCTAT AATGACCCCG
     3481 AAGCAGGGTT ATGCAGCGGA AAACGGCCAC GATGCGTCCG GCGTAGAGGA TCTGAAGATC
     3541 AGCAGTTCAA CCTGTTGATA GTACGTACTA AGCTCTCATG TTTCACGTAC TAAGCTCTCA
     3601 TGTTTAACGT ACTAAGCTCT CATGTTTAAC GAACTAAACC CTCATGGCTA ACGTACTAAG
     3661 CTCTCATGGC TAACGTACTA AGCTCTCATG TTTCACGTAC TAAGCTCTCA TGTTTGAACA
     3721 ATAAAATTAA TATAAATCAG CAACTTAAAT AGCCTCTAAG GTTTTAAGTT TTATAAGAAA
     3781 AAAAAGAATA TATAAGGCTT TTAAAGCTTT TAAGGTTTAA CGGTTGTGGA CAACAAGCCA
     3841 GGGATGTAAC GCACTGAGAA GCCCTTAGAG CCTCTCAAAG CAATTTTGAG TGACACAGGA
     3901 ACACTTAACG GCTGACATGG GAATTAGCTT CACGCTGCCG CAAGCACTCA GGGCGCAAGG
     3961 GCTGCTAAAG GAAGCGGATA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC
     4021 CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC
     4081 TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGT
//